Quantification in Dragonfly is based on the concept of a multi-ROI, in which each group of connected voxels is labeled as a distinct object. Whether or not two voxels are connected is defined by their connectivity, that is, which voxels are neighbors and which are not.
With the Object Analysis module for Dragonfly, you can measure and quantify the objects within multi-ROIs, such as fibers, vesicles, and puncti. Using filtering parameters — such as volume, surface area, Phi and Theta angles, and the maximum, minimum, and mean values of an object — you can then classify objects that share specific characteristics. Additional options let you split and merge objects, as well as to export your analysis results.